美国戴安Dionex ProPac HIC Protein and Peptide Column简要描述：
戴安公司生产的仪器主要有离子色谱仪、高效液相色谱仪、快速溶剂萃取仪和氨基酸直接分析仪。其中离子色谱仪凭借着雄厚的技术优势和方法已广泛地应用于各个领域，其市场占有率达80%。与离子色谱相关的文章有90%均出自戴安公司生产的离子色谱仪。 戴安公司的快速溶剂萃取仪15分钟之内仅用15毫升溶剂既可完成对10g样品的萃取，该技术已在环境、药物、食品和聚合物工业等领域得到广泛应用。
美国戴安Dionex ProPac HIC Protein and Peptide Column
Hydrophobic Interaction Chromatography Columns for the Separation of Large Biomolecules
Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.
Based on 5 µm ultrahigh purity spherical silica gel particles, with 300 Å poresProteins separated under non-denaturing conditionsHigh protein loading capacity for protein purification applicationsProprietary multidentate silica bonding for enhanced hydrolytic stabilityHigh-resolution HPLC separation of proteins and peptidesWide range of applications
The ProPac® HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.
| ProPac HIC Columns Specifications |
|---|
| Protein Loading Capacity | 340 mg Lysozyme per 7.8 x 75 mm column |
| Starting Material | Ultrapure Spherical Silica |
| Particle Size | 5µm |
| Pressure Limit | 4,000 psi |
| Column Construction | Stainless Steel |
| Phase | HIC |
| Average Pore Diameter | 300Å |
| Endcapped | No |
| Phase Compatibility | 2 M Ammonium sulfate/ phosphate salts, organic solvents for cleanup |
| pH range | pH 2.5–7.5 |
| Temperature Limit | < 40°C |
| Analytical Columns |
|---|
| ProPac HIC-10 Column (2.1 x 100 mm) | 063653 |
| ProPac HIC-10 Column (4.6 x 100 mm) | 063655 |
| ProPac HIC-10 (4.6 x 250mm) | 074197 |
| ProPac HIC-10 Column (7.8 x 75 mm) | 063665 |
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