QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒12381
2023-12-12
QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
产品型号：12381
金山科研平台授权一级代理，产品询价请咨询微信：jinshanbio
QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒EndoFree Plasmid Kits基于阴离子交换技术，可快速制备不含内毒素的质粒DNA。QIAfilter Cartridges过滤快速澄清裂解液。制备的DNA纯度超过两次CsCl梯度离心法所得DNA的纯度，适合超转染级纯的应用。EndoFree Plasmid Buffer Set可用于制备10次m
金山科研平台授权代理，咨询微信：jinshanbio
QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
纯化得到至多10mg的无内毒素高转染级纯质粒或柯斯质粒DNA
纯化得到的DNA内毒素水平低于0.1 EU/µg
纯化流程快，同时去除内毒素
获得高产量、高拷贝质粒DNA
使用LyseBlue，获得的裂解和高产量的DNA
QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
质粒纯化方法与转染效率。
使用两种方法独立制备pRSVcat，分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中，及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit（100％）制备的DNA的转染效率表示。
质粒纯化方法与转染效率。
使用两种方法独立制备pRSVcat，分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中，及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit（100％）制备的DNA的转染效率表示。
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| EndoFree Plasmid Giga Kit (5) | 8080 | 12391 | 5 |
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| QIAGEN Plasmid Plus Midi Sample Kit (5) | 723 | 12941 | |
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| QIArack | 1250 | 19015 | |
| Plasmid Buffer Set | 1410 | 19046 | |
| EndoFree Plasmid Buffer Set | 7430 | 19048 | |
| Buffer P1 (500 ml) | 811 | 19051 | 500 |
| Buffer P2 (500ml) | 811 | 19052 | 500 |
| Buffer P3 (500ml) | 811 | 19053 | 500 |
| Buffer QBT (1000ml) | 1410 | 19054 | 1000 |
| Buffer QC (1000ml) | 1410 | 19055 | 1000 |
| Buffer QF (1000ml) | 1410 | 19056 | 1000 |
| Genomic DNA Buffer Set | 2080 | 19060 | 75 |
| Buffer QG (250 ml) | 666 | 19063 | 250 |
| Buffer N3 (500ml) | 1060 | 19064 | 500 |
| Buffer PE (concentrate, 100ml) | 1060 | 19065 | 100 |
| Buffer PB (500ml) | 1060 | 19066 | 500 |
| Buffer AW/AW2 (concentrate, 324 ml) | 1220 | 19072 | 324 |
| Buffer AVL (155 ml) | 1900 | 19073 | |
| Buffer AL | 1220 | 19075 | 1 |
| Buffer ATL (200ml) | 1220 | 19076 | 200 |
| Buffer AE (240ml) | 1220 | 19077 | 1 |
| Buffer AW1 (concentrate, 242 ml) | 1220 | 19081 | 1 |
| Buffer ASL (4 x 140 ml) | 1370 | 19082 | |
| Buffer PM (500ml) | 837 | 19083 | 500 |
| Buffer EB (250ml) | 423 | 19086 | 250 |
| Reagent DX | 663 | 19088 | 1 |
| Buffer RPW (125ml) | 598 | 19090 | 125 |
| Buffer MTL (54 ml) | 466 | 19112 | 54 |
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| QIAGEN Proteinase K (10 ml) | 4200 | 19133 | 1 |
| QIAGEN Protease (7.5 AU) | 996 | 19155 | |
| QIAGEN Protease (30 AU) | 3200 | 19157 | 30 |
| Collection Tubes (2-ml) | 1830 | 19201 | |
| VacConnectors (500) | 1820 | 19407 | |
| VacValves (24) | 1250 | 19408 | |
| QIAvac 24 Plus | 4300 | 19413 | |
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| QIAvac Connecting System | 13950 | 19419 | |
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| Collection Microtube Caps (120 x 8) | 439 | 19566 | |
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| QIAEX II Gel Extraction Kit (150) | 2340 | 20021 | 150 |
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| Strep-Tactin Superflow Plus Cartrdg(5ml) | 6370 | 30060 | 5 |
| Ni-NTA Agarose (25 ml) | 3850 | 30210 | 25 |
| Ni-NTA Agarose (100 ml) | 13370 | 30230 | 100 |
| Ni-NTA Agarose (500 ml) | 56230 | 30250 | 500 |
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| EpiTect MethyLight PCR +ROX Kit (200) | 10600 | 59496 | 200 |
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| EpiTect Control DNA, unmethylated (100) | 2730 | 59665 | 100 |
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