Talin-1 Antibody （YQ-16）

产品型号：santacruz SC-81805

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mouse monoclonal IgG3 provided at 100 µg/mlrecommended for detection of Talin-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

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SANTA CRUZ BIOTECHNOLOGY, INC.Talin-1 （YQ-16）: sc-81805Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.comBACKGROUNDFocal adhesions were identified as areas within the plasma membrane oftissue culture cells that adhere tightly to the underlying substrate. In vivo,these regions are involved in the adhesion of cells to the extracellular matrix.Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are componentsof a protein complex that links the Actin network to the plasmamembrane. Vinculin binding sites have been identified on other cytoskeletalproteins, including Talin-1 and α-actinin. In addition, vinculin, Talin-1, Talin-2and α-actinin each contain Actin binding sites. Expression of vinculin, Talin-1and Talin-2 have been shown to be affected by the level of Actin expression.α-actinin has been shown to link Actin to integrins in the plasma membranethrough interactions with the vinculin and Talin complex or by a direct interactionwith integrin. Talin-2 is similar to Talin-1 but shows distinct patterns ofexpression and cannot compensate for the loss of Talin-1.REFERENCES1. Burridge, K., et al. 1988. Focal adhesions: transmembrane junctionsbetween the extracellular matrix and the cytoskeleton. Annu. Rev. CellBiol. 4: 487-525.2. Gilmore, A.P., et al. 1992. Further characterization of the Talin-binding sitein the cytoskeletal protein vinculin. J. Cell Sci. 103: 719-731.3.Wood, C.K., et al. 1994. Characterisation of the paxillin-binding site andthe C-terminal focal adhesion targeting sequence in vinculin. J. Cell Sci.107: 709-717.4. Gluck, U., et al. 1994. Modulation of α-actinin levels affects cell motilityand confers tumorigenicity on 3T3 cells. J. Cell Sci. 107: 1773-1782.5. Schevzov, G., et al. 1995. Impact of Actin gene expression on vinculin,Talin, cell spreading, and motility. DNA Cell Biol. 14: 689-700.6. Hemmings, L., et al. 1996. Talin contains three Actin-binding sites each ofwhich is adjacent to a vinculin-binding site. J. Cell Sci. 109: 2715-2726.7. Gilmore, A.P., et al. 1996. Regulation of vinculin binding to Talin and Actinby phosphatidyl-inositol-4-5-bisphosphate. Nature 381: 531-535.CHROMOSOMAL LOCATIONGenetic locus: TLN1 （human） mapping to 9p13.3; Tln1 （mouse） mapping to4 B1.SOURCETalin-1 （YQ-16） is a mouse monoclonal antibody raised against recombinantTalin-1 of human origin.PRODUCTEach vial contains 100 μg IgG3 in 1.0 ml PBS with < 0.1% sodium azide and0.1% gelatin.STORAGEStore at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required.APPLICATIONSTalin-1 （YQ-16） is recommended for detection of Talin-1 of mouse, rat andhuman origin by Western Blotting （starting dilution 1:200, dilution range1:100-1:1000）, immunoprecipitation [1-2 μg per 100-500 μg of total protein（1 ml of cell lysate）], immunofluorescence （starting dilution 1:50, dilutionrange 1:50-1:500）, immunohistochemistry （including paraffin-embeddedsections） （starting dilution 1:50, dilution range 1:50-1:500） and solid phaseELISA （starting dilution 1:30, dilution range 1:30-1:3000）.Suitable for use as control antibody for Talin-1 siRNA （h）: sc-36610, Talin-1siRNA （m）: sc-36611, Talin-1 shRNA Plasmid （h）: sc-36610-SH, Talin-1 shRNAPlasmid （m）: sc-36611-SH, Talin-1 shRNA （h） Lentiviral Particles: sc-36610-Vand Talin-1 shRNA （m） Lentiviral Particles: sc-36611-V.Molecular Weight of Talin-1: 230 kDa.Positive Controls: RAW 264.7 whole cell lysate: sc-2211.RECOMMENDED SECONDARY REAGENTSTo ensure optimal results, the following support （secondary） reagents arerecommended: 1） Western Blotting: use goat anti-mouse IgG-HRP: sc-2005（dilution range: 1:2000-1:32,000） or Cruz Marker™ compatible goat antimouseIgG-HRP: sc-2031 （dilution range: 1:2000-1:5000）, Cruz Marker™Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2） Immunoprecipitation:use Protein A/G PLUS-Agarose: sc-2003 （0.5 ml agarose/2.0 ml）.3） Immunofluorescence: use goat anti-mouse IgG-FITC: sc-2010 （dilutionrange: 1:100-1:400） or goat anti-mouse IgG-TR: sc-2781 （dilution range:1:100-1:400） with UltraCruz™ Mounting Medium: sc-24941. 4） Immunohistochemistry:use ImmunoCruz™: sc-2050 or ABC: sc-2017 mouse IgGStaining Systems.DATASELECT PRODUCT CITATIONS1. Ah Kioon, M.D., et al. 2010. Adrenomedullin increases fibroblast-like synoviocyteadhesion to extracellular matrix proteins by upregulating integrinactivation. Arthritis Res. Ther. 12: R190.RESEARCH USEFor research use only, not for use in diagnostic procedures.

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