R&D代理Recombinant Rat EGF重组鼠表皮生长因子RD供应*

产品型号：3214-EG-100

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此类产品还有人的表皮细胞生长因子，上海利民实业有限公司是华东区RD代理，咨询产品 纤细说明书见金山科研平台授权代理，咨询微信：jinshanbio

金山科研平台授权代理，咨询微信：jinshanbio

DESCRIPTIONSource E. coliderivedAsn974Arg1026,with an NterminalMetAccession # P07522NterminalSequenceAnalysisMetPredicted MolecularMass6.3 kDaSPECIFICATIONSActivity Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. （1991） Proc. Natl. Acad. Sci. USA88:415.The ED50 for this effect is typically 1060pg/mL.Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.Purity >97%, by SDSPAGEunder reducing conditions and visualized by silver stain.Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.PREPARATION AND STORAGEReconstitution Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.Stability & Storage Use a manual defrost freezer and avoid repeated freezethawcycles.l 12 months from date of receipt, 20to 70°C as supplied.l 1 month, 2 to 8 °C under sterile conditions after reconstitution.l 3 months, 20to 70°C under sterile conditions after reconstitution.BACKGROUNDEpidermal growth factor （EGF） is the founding member of the EGF family that also includes TGFα,amphiregulin （AR）, betacellulin （BTC）, epiregulin （EPR）, heparinbindingEGFlikegrowth factor（HBEGF）,epigen, and the neuregulins （NRG）1through 6（1）. Members of the EGF family share a structural motif, the EGFlikedomain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues （2）. All EGF familymembers are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins arereleased from the cell surface by regulated proteolysis （1）. The 1133 amino acid （aa） rat EGF precursor contains nine EGF domains and nine LDLR class B repeats.The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region （3）. Mature rat EGF shares70% and 77% aa sequence identity with mature human and mouse EGF, respectively. EGF is present in various body fluids, including blood, milk, urine, saliva,seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid （4）. Four ErbB （HER） family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 andErbB4, mediate responses to EGF family members （5）. These receptors undergo a complex pattern of ligand induced homoorheterodimerizationto transduce EGFfamily signals （6, 7）. EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results inautophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules （5, 8）. Biological activities ascribed toEGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.References:1. Harris, R.C. et al. （2003） Exp. Cell Res. 284:2.2. Carpenter, G. and Cohen, S. （1990） J. Biol. Chem. 265:7709.3. Saggi, S.J. et al. （1992） DNA Cell Biol. 11:481.4. Carpenter, G. and Zendegui, J.G. （1986） Exp. Cell Res. 164:1.5. Jorissen, R.N. et al. （2003） Exp. Cell Res. 284:31.6. Gamett, D.C. et al. （1997） J. Biol. Chem. 272:12052.7. Qian, X. et al. （1994） Proc. Natl. Acad. Sci. 91:1500.8. Qian, X. et al. （1999） J. Biol. Chem. 274:574.Recombinant Rat EGFCatalog Number: 3214-EGwww.