DAKO一抗稀释液Antibody Diluent, Background Reducing

产品型号：Dako s3022 Dako代理

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for dilution of antibodies which tend to give high, non-specific background staining in immunohistochemical procedures.Diminishes background staining while maintaining adequate,specific staining

金山科研平台授权代理，咨询微信：jinshanbio

DAKO一抗稀释液Antibody Diluent, Background Reducing

*的一抗稀释液

DAKO一抗稀释液Antibody Diluent, Background Reducing说明：

Intended use For In Vitro diagnostic use.This product is intended for use as a diluent in the preparation of primary antibodies and negative controlreagents. It is to be used with antibodies and/or antigens that yield high background in immunohistochemical（IHC） staining procedures.Refer to the “General Instructions for Immunohistochemical Staining” or the Detection System “Instructions” ofIHC procedures for: （1） Principle of Procedure, （2） Materials Required, Not Supplied, （3） Storage, （4） SpecimenPreparation, （5） Staining Procedure, （6） Quality Control, （7） Troubleshooting, （8） Interpretation of Staining,（9） General Limitations.Reagent provided 0.05 mol/L Tris-HCl buffer containing 0.1% Tween, stabilizing proteins to reduce background, and 0.015 mol/Lsodium azide.Precautions 1. For professional users.2. This product contains sodium azide （NaN3）, a chemical highly toxic in pure form. At product concentrations,though not classified as hazardous, build-ups of NaN3 may react with lead and copper plumbing to formhighly explosive metal azides. Upon disposal, flush with large volumes of water to prevent azide build-up inplumbing.1,23. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.4. Unused solution should be disposed of according to local, State and Federal regulations.Reactivity For demonstration of tissue antigens by IHC, optimal staining performance is dependent on the correct dilutionof the primary antibody. The interaction between antibodies and epitopes consists mainly of relatively weakforces such as van der Waals forces, electrostatic forces and hydrophobic forces, which are influenced by pHand ionic strength. Consequently, to assure optimal performance of the antibody with the least amount ofbackground, the choice of diluent is important.Applications This product provides an appropriay buffered solution for the dilution of both polyclonal and monoclonalantibodies and for the preparation of negative control reagents in IHC. This product is particularly useful forreducing background staining while maintaining specific reactivity. Antibody Diluent with Background ReducingComponents can also be used to stabilize diluted antibodies when stored at 2–8°C. This diluent should bebrought to room temperature and mixed thoroughly prior to use.Limitations Tissue staining results are dependent upon the handling and processing of the tissue prior to staining.Aldehyde-containing fixatives, such as formalin and glutaraldehyde frequently contribute to increasedbackground staining. Improper fixation, freezing, thawing, washing, drying, heating or sectioning may produceartifacts, antibody trapping or false-negative results. Also, false-positive staining may be caused by crossreactivityof other IHC staining reagents to e.g. endogenous alkaline phosphatase, endogenous peroxidase,pseudoperoxidase, endogenous avidin-binding activity and/or by nonspecific reaction with necrotic ordegenerated cells.Storage Store at 2–8°C. Do not freeze.

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