EPTOMICS代理Atg12 （ATG12） antibody Eptomics公司抗体供应商3473-1
EPTOMICS代理Atg12 （ATG12） antibody Eptomics公司抗体供应商
产品型号：3473-1
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EPTOMICS代理Atg12 （ATG12） antibody Eptomics公司抗体供应商Recommended ApplicationsWB, IHC, ICC, IP, FC
金山科研平台授权代理，咨询微信：jinshanbio
EPTOMICS代理Atg12 （ATG12） antibody Eptomics公司抗体供应商
Rabbit Monoclonal Antibody
Description
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy （1）. It is essential for Atg12 conjugation, and the ATG12-ATG5 conjugate is necessary to form autophagic vesicles （2）. The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required （3）.
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000-10,000IHC: 1:50-100ICC: 1:100-250IP: 1:10-100FC: 1:10-100
Species Reactivity *
Human
*Species cross-reactivity is based on WB analysis
Products Data
| Western blot analysis on （A） THP-1, （B） HepG2, （C） Raji, and （D） fetal kidney lysates using anti-Atg12 RabMAb （cat. #3473-1）. | Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 RabMAb （cat. #3473-1）. | Western blot analysis on （A） Atg12 transfected 293T and （B） 293T lysates using anti-Atg12 RabMAb （cat. #3473-1）. |
Specificity
A synthetic peptide corresponding to residues in human Atg12 was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
ATG12 antibody, APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Ubiquitin-like protein ATG12 antibody, Autophagy-related protein 12 antibody
Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.2. Phillips AR et al. Genetics. 2008 Mar;178（3）:1339-53.3. Williams RA et al. Autophagy. 2009 Feb;5（2）:159-72.
| Product Name | Catalog # |
|---|---|
| Goat Anti-Rabbit IgG （H+L） HRP | 3053-1 |
| Goat Anti-Rabbit IgG polymerized HRP | 3051-1 |
| Cell Lysis Buffer | 3500-1 |
| Goat Anti-Rabbit IgG HRP Antibody | 3056-1 |
| Goat Anti-Rabbit IgG DyLight® 405 | 3063-1 |
| Goat Anti-Rabbit IgG DyLight® 488 | 3064-1 |
| Goat Anti-Rabbit IgG DyLight® 550 | 3065-1 |
| Goat Anti-Rabbit IgG DyLight® 594 | 3066-1 |
| Goat Anti-Rabbit IgG DyLight® 633 | 3067-1 |
| Goat Anti-Rabbit IgG DyLight® 650 | 3068-1 |
EPTOMICS代理Atg12 （ATG12） antibody Eptomics公司抗体供应商
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