M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

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M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF也被称为CSF1细胞因子，巨噬细胞的存活，增殖和分化（1）主要监管机构纤细说明书在产品金山科研平台授权代理，咨询微信：jinshanbio

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M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

DESCRIPTIONSource E. coliderivedLys33Glu262,with an NterminalMetAccession # Q3U4F9NterminalSequenceAnalysisMetStructure / Form DisulfidelinkedhomodimerPredicted MolecularMass26 kDa （monomer）SPECIFICATIONSSDSPAGE29 kDa, reducing conditionsActivity Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. Halenbeck, R. et al. （1989）Biotechnology 7:710.The ED50 for this effect is typically 0.5-3 ng/mL.Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.Purity >97%, by SDSPAGEunder reducing conditions and visualized by silver stain.Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. See Certificate of Analysis for details.PREPARATION AND STORAGEReconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.Stability & Storage Use a manual defrost freezer and avoid repeated freezethawcycles.l 12 months from date of receipt, 20to 70°C as supplied.l 1 month, 2 to 8 °C under sterile conditions after reconstitution.l 3 months, 20to 70°C under sterile conditions after reconstitution.BACKGROUNDMCSF,also known as CSF1,is a fourαhelicalbundlecytokine that is the primary regulator of macrophage survival, proliferation and differentiation （1 3）.MCSFis also essential for the survival and proliferation of osteoclast progenitors （1, 4）. MCSFalso primes and enhances macrophage killing of tumor cells andmicroorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis （2, 3）. MCSFincreases duringpregnancy to support implantation and growth of the decidua and placenta （5）. Sources of MCSFinclude fibroblasts, activated macrophages, endometrial secretoryepithelium, bone marrow stromal cells and activated endothelial cells （1 5）.The MCSFreceptor （cfms）transduces its pleotropic effects and mediates itsendocytosis. MCSFmRNAs of various sizes occur （3 9）.Full length mouse MCSFtranscripts encode a 520 amino acid （aa） type I transmembrane （TM） protein witha 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces twoproteolytically cleaved, secreted dimers. One is an NandOglycosylated86 kDa dimer, while the other is modified by both glycosylation and chondroitinsulfateproteoglycan （PG） to generate a 200 kDa subunit. Although PGmodifiedMCSFcan circulate, it may be immobilized by attachment to type V collagen （8）. Shortertranscripts encode M CSFthat lacks cleavage and PG sites and produces an Nglycosylated68 kDa TM dimer and a slowly produced 44 kDa secreted dimer （7）.Although forms may vary in activity and halflife,all contain the Nterminal150 aa portion that is necessary and sufficient for interaction with the M CSFreceptor（10, 11）. The first 229 aa of mature mouse MCSFshares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human MCSF,respectively （12, 13）. Human MCSFis active in the mouse, but mouse MCSFis reported to be speciesspecific.M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

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