fermentasRNA酶抑制剂RiboLock RNase InhibitorEO0381
fermentasRNA酶抑制剂RiboLock RNase Inhibitor
产品型号：EO0381
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fermentasRNA酶抑制剂RiboLock RNase Inhibitor应用范围广，适合多种使用条件。保护RNA，使其免受降解。Z高有效温度达55°C规格单位：2500U 4*2500U
金山科研平台授权代理，咨询微信：jinshanbio
fermentasRNA酶抑制剂RiboLock RNase Inhibitor
Description
Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.
HighlightsPerforms under a wide range of reaction conditions
Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)
NoteDTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity.
Specifications
| CategoryName | |
| Concentration | 40U/µL |
| Definition of Activity Unit | One unit of the RiboLock RNase Inhibitor inhibits the activity of 5ng RNase A by 50%. Inhibitor activity is assayed in the following mixture: 100mM Tris-HCl (pH7.5), 1.2mM EDTA, 0.1mg/mL BSA, 100ng/mL RNase A, 0.1mg/mL [3H]-RNA, 50mg/mL yeast RNA, 8mM DTT. |
| Hazardous | No |
| Hazardous: | No |
| Inactivation | Inactivated by heating at 75°C for 10min. Residual activity detectable after 10min heating at 70°C. |
| Inhibition | Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound. |
| Molecular Weight | 49.6kDa monomer |
| Quality Control | The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests. Functionally tested in RNA and cDNA synthesis. |
| Shelf Life: | |
| Shipping Condition: | |
| Shipping Information | |
| Source | E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor. |
| SpecificationName | |
| SpecificationValue | |
| Storage Buffer | The enzyme is supplied in 20mM HEPES-NaOH (pH7.5), 50mM NaCl, 8mM DTT, 0.03%v/v) ELUGENT Detergent and 50%(v/v) glycerol. |
| Storage Condition | -20 C |
Storage Condition: |
fermentasRNA酶抑制剂RiboLock RNase Inhibitor *
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